auriga compact focused ion beam scanning electron microscopy (fib-sem)  (Carl Zeiss)

Journal: Microbiome

Article Title: Linking morphology, genome, and metabolic activity of uncultured magnetotactic Nitrospirota at the single-cell level

doi: 10.1186/s40168-024-01837-6

Figure Lengend Snippet: The pipeline developed in this study. For the target-specific cell sorting and mini-metagenomics, different numbers of magnetotactic bacteria (MTB) cells were sorted with a micromanipulation system (Step 1), which were then lysed and used as the template of whole genome amplification (Step 2). After sequencing, assembly, and binning, the draft genome of the MTB population was obtained (Step 3). Genome annotation and subsequent analysis (including phylogeny analysis, metabolism analysis) were then performed (Step 4). For the NanoSIMS-based isotopic analysis, the stable-isotope incubated MTB cells were first magnetically enriched (Step 1), then characterized by fluorescence in situ hybridization (FISH) (Step 2) and focused ion beam scanning electron microscope (FIB-SEM) (Step 3), and finally analyzed by NanoSIMS at the single-cell level (Step 4)

Article Snippet: The silicon wafer with enriched MTB cells was placed in a scanning electron microscope (SEM) equipped with a focused ion beam (FIB) system (Zeiss Auriga Compact FIB-SEM, Germany).

Techniques: FACS, Bacteria, Micromanipulation, Whole Genome Amplification, Sequencing, Incubation, Fluorescence, In Situ Hybridization, Microscopy

Journal: Microbiome

Article Title: Linking morphology, genome, and metabolic activity of uncultured magnetotactic Nitrospirota at the single-cell level

doi: 10.1186/s40168-024-01837-6

Figure Lengend Snippet: A combination of FISH, FIB-SEM, and NanoSIMS analyses. a–c LHC-1 cells were mixed with E. coli cells and incubated with NaH 13 CO 3 for 1 h and then fixed and dried on a silicon wafer. Thereafter, the FISH experiment was conducted using a universal bacterial probe EUB338 ( a ) and an LHC-1-specific probe BTC19 ( b ), and the bacteria were imaged with an Olympus Optical BX51 fluorescence microscope. c A merged image of a and b . The white arrowheads point to E. coli cells in a – c . d SEM image of the same region of interest (ROI) of a to c , which was acquired at an accelerating voltage of 5 kV with a 6-mm working distance. The LHC-1 cells can be easily distinguished from E. coli due to their disparity in sizes, and the correspondence with the LHC-1-specific probe BTC19 in b . e Pt deposition (yellow arrowheads) on the ROI with FIB-SEM as markers for NanoSIMS imaging. The ROI analyzed using NanoSIMS is outlined as white rectangular. f NanoSIMS image of 12 C − of the same ROI

Article Snippet: The silicon wafer with enriched MTB cells was placed in a scanning electron microscope (SEM) equipped with a focused ion beam (FIB) system (Zeiss Auriga Compact FIB-SEM, Germany).

Techniques: Incubation, Bacteria, Fluorescence, Microscopy, Imaging

Journal: Scientific Reports

Article Title: Effect of processing parameters on texture and variant selection of as-built 300 maraging steel processed by laser powder bed fusion

doi: 10.1038/s41598-022-19835-9

Figure Lengend Snippet: Martensite EBSD maps of the EOS—40 μm—CW laser condition, taken on the ( a , b ) transverse section and ( c , d ) longitudinal section. Pixels are colored according to their Inverse Pole Figures (IPF) color, corresponding to the building direction—perpendicular to the map for ( a , b ) and horizontal for ( c , d ). Prior austenite grain boundaries are superimposed on the maps.

Article Snippet: The microstructures located in the middle, in both the transverse (T) and longitudinal (L) section of the sample built in the EOS M270 machine—approximate at a height of 5 mm—were also characterized by Electron Backscattered Diffraction (EBSD) in a Zeiss Auriga Compact Focused Ion Beam-Scanning Electron Microscope (FIB-SEM), operating at 20 kV. includes a sketch showing the location of these two sections.

Techniques:

Journal: Scientific Reports

Article Title: Effect of processing parameters on texture and variant selection of as-built 300 maraging steel processed by laser powder bed fusion

doi: 10.1038/s41598-022-19835-9

Figure Lengend Snippet: ( a , c , d , g , i , k , m ) \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\varphi_{2}$$\end{document} φ 2 = 0° and ( b , d , f , h , j , l , n ) \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\varphi_{2}$$\end{document} φ 2 = 45° ODF sections corresponding to the martensitic matrix of the middle ( e – l ), top ( a , b ) and bottom ( c , d ) layers of the samples and to a sketch depicting some important texture components or textures that are named in the main text ( m , n ). Data corresponds to martensite data measured on the EOS—40 μm—CW laser condition by ( a – d ) XRD and ( e – h ) EBSD (transverse T and longitudinal L sections) and to reconstructed austenite data obtained from the EBSD data ( i – l ). Intensities correspond to the color bar on the right-hand side, where the units are multiples of random distribution (MRD).

Article Snippet: The microstructures located in the middle, in both the transverse (T) and longitudinal (L) section of the sample built in the EOS M270 machine—approximate at a height of 5 mm—were also characterized by Electron Backscattered Diffraction (EBSD) in a Zeiss Auriga Compact Focused Ion Beam-Scanning Electron Microscope (FIB-SEM), operating at 20 kV. includes a sketch showing the location of these two sections.

Techniques:

Journal: Scientific Reports

Article Title: Effect of processing parameters on texture and variant selection of as-built 300 maraging steel processed by laser powder bed fusion

doi: 10.1038/s41598-022-19835-9

Figure Lengend Snippet: Variant selection study, where ( a , b ) represent the area percentages corresponding to each variant number for the EOS—40 μm—CW laser condition. Data corresponds to the EBSD maps taken on the ( a ) transverse section and ( b ) longitudinal sections. Dashed lines represent the area percentage that would be expected without variant selection. The grey areas show which packets the variants belong to, whereas the marker colors change depending on the \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$BG$$\end{document} BG to which the variant belongs. Subfigures ( c , d ) show the Theoretical pole figures (PF) corresponding to ( c ) the prior austenite and to ( d ) the resultant martensite, calculated by applying the orientation relationship corresponding to all variants (in red) or only the selected variants (in blue). PFs correspond to the BD.

Article Snippet: The microstructures located in the middle, in both the transverse (T) and longitudinal (L) section of the sample built in the EOS M270 machine—approximate at a height of 5 mm—were also characterized by Electron Backscattered Diffraction (EBSD) in a Zeiss Auriga Compact Focused Ion Beam-Scanning Electron Microscope (FIB-SEM), operating at 20 kV. includes a sketch showing the location of these two sections.

Techniques: Variant Assay, Selection, Marker

Journal: Scientific Reports

Article Title: Effect of processing parameters on texture and variant selection of as-built 300 maraging steel processed by laser powder bed fusion

doi: 10.1038/s41598-022-19835-9

Figure Lengend Snippet: Correlative ( a , d ) SEM ( b , c , e , f ) EBSD results corresponding to the transverse ( a – c ) and longitudinal ( d – f ) sections of the EOS condition—40 μm—CW laser, where the SD and the BD are indicated by a grey and a black arrow, respectively, and where the EBSD orientations are colored according to their IPF—BD color. The EBSD data correspond to ( b , e ) the bcc phase and ( c , f ) the corresponding reconstructed prior fcc phase. The dashed black lines represent the MP boundary and the arrows represent the different colony growth directions in given prior fcc grain. Each of the arrows is identified by an ID.

Article Snippet: The microstructures located in the middle, in both the transverse (T) and longitudinal (L) section of the sample built in the EOS M270 machine—approximate at a height of 5 mm—were also characterized by Electron Backscattered Diffraction (EBSD) in a Zeiss Auriga Compact Focused Ion Beam-Scanning Electron Microscope (FIB-SEM), operating at 20 kV. includes a sketch showing the location of these two sections.

Techniques: